However, crude extracts from other terminalia species have previously been reported as a glucosidase inhibitors anam et al. The mechanism of alphaglucosidase inhibition in the. I also dedicate this thesis to my son, joshua, who was born in the course of my program. M, while that of the reference drugs acarbose and 1. Glucosidase definition of glucosidase by medical dictionary. Antidiabetic and antiobesity activities of cocoa theobroma cacao via physiological enzyme inhibition caroline mary ryan thesis submitted to the faculty of the virginia polytechnic institute and state university in partial fulfillment of the requirements for the degree of master of science in life science in food science and technology. Dubois a thesis submitted in partial fulfillment of the requirements for a degree with honors food science and human nutrition the honors college university of maine may 2014 advisory committee. To better understand the complementary activities and mechanism of inhibition of these intestinal glucosidases, this thesis aims to characterize the individual n. Ic 50 value of acarbose in iaghmaltase assay system, 340430 nm. Pdf diabetes mellitus dm is recognized as a serious global health problem. Of the substances tested in our assay, chebulagic acid is the most efficient inhibitor of. The modes of inhibition of both enzymes was determined using lineweaverburke plot. Antidiabetic potentials of phenolic enriched chilean.
Alpha glucosidase inhibitor an overview sciencedirect. In the latter context, the glucose tolerant bgs are of particular interest. Pdf numerous metabolic disorders and diseases associated with the glucosidase enzyme activity. These bgs often show peculiar kinetics, including inhibitory. The presence of peanut skin in mwp rendered up to 9. The method is based on the specific recognition between 1,4phenylenediboronic acid pdba and 4aminophenyl. The acetone extract of picralima nitida displayed the most effective inhibition of both.
In vitro studies on alpha amylase and alpha glucosidase. This is an easy, quick and highthroughput capable kit that can measure 0. In all tests, acarbose, a pharmacological inhibitor of. Noncompetitive inhibition is a type of enzyme inhibition where the inhibitor reduces the activity of the enzyme and binds equally well to the enzyme whether or not it has already bound the substrate the inhibitor may bind to the enzyme whether or not the substrate has already been bound, but if it has a higher affinity for binding the enzyme in one state or the other, it is called a mixed. Glucosidase hydrolyzes carbohydrates by acting on terminal, nonreducing 1 4linked dglucose residues with the release of dglucose. They constitute an important group of enzymes with biotechnological interest like supporting cellulases in degradation of lignocellulose to fermentable sugars. Alphaglucosidase inhibitors agis are oral antidiabetic drugs used for diabetes mellitus type 2 that work by preventing the digestion of carbohydrates such as starch and table sugar. Characterizing the inhibition of mammalian intestinal. Glucosidase hydrolyzes the substrate mix to release the pnitrophenol that can be measured colorimetrically od 410 nm. One mg of glucosidase saccharomyces cerevisiae, sigmaaldrich, usa was dissolved in 100 ml of phosphate buffer ph 6. The results of the both enzyme inhibition activity was found in a dosedependent manner, the strongest activity in case of.
Agathis dramaga, bogor, indonesia 2biopharmaca research center, bogor agricultural university, jl. Biological and chemical studies of medicinal plants a. This honors thesis is brought to you for free and open access by. Department of pharmaceutical chemistry university of oslo may 2014. In niddm the delaying of glucose absorption after meal by inhibition of aglucosidase is bene. Honors college thesis the inhibition of aamylase and a. Bioactive constituents, vitamin analysis, antioxidant. However,ourfindingsrevealedthatnhydroxycinnamoyl amides with one tryptophan residue 3, 4wereinactive ic50 200gml, whereas the introduction of two tryptophan moieties in caffeic and sinapic acid amides 1, 2 pronouncedly exerted a higher glucosidase inhibition. Artocarpus heterophyllus showed the greater % inhibition of the alpha glucosidase enzyme compared to other plant extracts. Structural and inhibition studies of human intestinal glucosidases. Hence, further studies may throw light on the antidiabetic potential of a. Nowadays, alpha glucosidase inhibitors like acarbose.
The ability of berry extracts to inhibit alphaglucosidase in vitro. However, the observed inhibition values remained at high level, thus it could be concluded that the. Dglucopyranoside that is hydrolyzed specifically by. The present study indicated that artocarpus heterophyllus could be useful in. A thesis submitted in partial fulfillment of the requirements for the degree of master.
Antidiabetic potentials of phenolic enriched chilean potato and select herbs of apiaceae and lamiaceae families a thesis presented by fahad saleem submitted to the graduate school of the university of massachusetts amherst in partial fulfillment of the requirements for the degree of master of science september 2010 molecular and cellular biology. The inhibition of glucosidase activity was determined using the modified published method. The mechanism of alphaglucosidase inhibition in the management of diabetes. The ability of berry extracts to inhibit alphaglucosidase. Screening of glucosidase inhibitory activity from some. The present study intends to screen novel alpha amylase and alpha glucosidase inhibitors from natural sources like plants in order to minimize the toxicity and side effects of the inhibitors currently used to control hyperglycemia. Glucosidase enzyme immediately prior to pipetting into reaction mixture, prepare.
M when compared with the standard drug acarbose ic 50 750. This thesis presents the results from in vitro studies on inhibition of. To better understand the complementary activities and mechanism of inhibition of these intestinal glucosidases, this thesis aims to characterize the individual n and cterminal mgam. Phenolics from all samples and fractions showed inhibition of alpha.
Pdf alpha glucosidase inhibitory effect and enzyme kinetics of. Kinematic analysis had previously shown root growth maintenance in the apical 3mm of seedlings under water deficits zhu, et al. However, pp4 and pp6 showed the best inhibition against. Glucosidase by the modified boehenger procedure ec 3. The highest inhibitory activity towards alphaglucosidase was found in the urtica urens hexane extract and the thymus vulgaris hexane extract 69. The development of the alphaglucosidase inhibitor acarbose provides a new approach in the management of diabetes. A new approach to the treatment of diabetes, obesity, and hyperlipoproteinaemia. Structural and inhibition studies of human intestinal.
Biological and chemical studies of medicinal plants. Chinese medicinal plant codonopsis pilosula yuanfeng zou thesis submitted for the degree of ph. Inhibition of these enzymes delay and in some cases halt carbohydrate digestion thus prolonging overall carbohydrate digestion time causing a reduction in the rate of glucose absorption and consequently reducing postprandial plasma glucose rise henfeld et al. The enzyme inhibition examinations were also carried out after the precipitation of tannins from the mixture causing only 7. The ability of berry extracts to inhibit alphaglucosidase in vitro by shannon e.
The dissertationthesis may not be published either in part in scholarly, scientific or. The sample absorbance values were read at 415 nm using the tecan. Carbohydrates are normally converted into simple sugars monosaccharides by alphaglucosidase enzymes present on cells lining the intestine, enabling monosaccharides to be absorbed through the intestine. Defects in glucosidase have been implicated in pompe disease and diabetes. The genus centaurea comprises of about 500 species, many of which have been. Therefore, the mechanism of glucosidase inhibition represents the pharmacological optimization of the dietary principle of delayed carbohydrate absorption. Glucosidase inhibition by miglitol in niddm patients. Immobilized agh iagh inhibition study by synthetic inhibitors acarbose and voglibose revealed that the magnitude of inhibition differed from that in the free agh fagh study. Field, for providing the compounds which made this thesis possible. The results of the present study indicate that, fraction 2 of a. I dedicate this thesis to god almighty who has enabled and seen me through this phase of my life. One of the strategies and methods adopted to cure diabetes mellitus involves the inhibition of carbohydrate digesting enzymes such as amylase and glucosidase in the gastrointestinal glucose. Purification of alphaglucosidase inhibitors from grape. Inhibitors of glucosidase delay the breaking down of carbohydrate in the small intestine and diminish the postprandial blood glucose excursion in a person suffering from diabetes.
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